QUANTIFOIL® Holey Carbon Supports for Cryo-EM


QUANTIFOIL® Holey Carbon supports are a flat film of carbon, 10-12 nm thick, on a cryo-electron microscopy (cryo-EM) grid. They provide a low-background sample support ideal for high-resolution cryo-EM data collection. The optimum grid for your project can be selected from a wide variety of hole shapes, sizes and spacings in combination with a range of base grid materials and mesh sizes. Grid options include standard square meshes as well as other arrangements and finder grid formats.

Quantifoil in peer-reviewed publications

Methods and review publications describing best practice in the field include:

Passmore and Russo. Specimen preparation for high-resolution cryo-EM. Methods Enzymol. 579: 510-86 (2016)

Drulyte et al. Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst D74: 560-571 (2018)

Koh et al. Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope. J Vis Exp 181 (2022)

CryoEM structures recently published in high impact journals include:

Arseni et al. TDP-43 forms amyloid filaments with a distinct fold in type A FTLD-TDP. Nature 620: 898-902 (2023)

Baggen et al. TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry. Cell 186:3427-3442 (2023)

Highly-cited articles from the first development of hihg-resolution cryoEM for biological specimens include:

Li et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat Methods. 10: 5840590 (2013)

Chen et al. High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy. Ultramicroscopy 135: 24-35 (2013)


QUANTIFOIL© Holey Carbon supports have been cited in approximately 6,000 peer-reviewed publications to date. 

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QUANTIFOIL® Grid categories


Ideal for high-resolution studies of biological macromolecules and other materials, round holes optimize the formation of a thin, even layer of ice to support a sample of interest. Select from our wide range of geometries depending on the characteristics of your sample and your imaging modality.

Explore circular holes


Often used for applications where larger carbon-free areas are desired, square grids have less carbon support. They can be used with an additional thin carbon layer where samples need continuous support.

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Multi A

With a repeating pattern of hole size, shape and spacing, Multi A foils are useful for particles of unpredictable size, for example liposomes, while the oval shape can aid in tomographic data collection.

Explore Multi A holes