A single grid with a range of hole geometries is useful when carrying out cryo-electron tomography (cryo-ET) or where the sample size may vary unpredictably (for example liposomes).The predefined pattern of hole sizes and shapes in QUANTIFOIL® Multi A offers this possibility while still enabling automated data collection, in contrast to the random arrangements of traditional holey or lacey supports.
Features include:
- Oval holes which appear round in tomographic sections with high tilt angles (~70°).
- Circular holes have diameters of 1 µm, 1.4 µm, and 2 µm.
- Elliptical holes measure 1 µm x 4 µm and 2 µm x 8 µm.
Did you know?
We can offer Multi A on finder grids and alternative meshes to maximise their utility for tomography and integrated methods applications.
Citations
Uses of the Multi A format include:
- Cryo-electron tomography, where the eliptical holes increase accessible tilt angle if aligned with the rotation axis. For example: Nedozralova et al. In situ cryo-electron tomography reveals local cellular machineries for axon branch development. J. Cell Biol. 221: e202106086 (2022).
- Larger particles and those of varying size. For example:
Koh et al. The structure of a 15-stranded actin-like filament from Clostridium botulinum. Nat. Commun. 10: 2856 (2019)
Rez et al. Damage-free vibrational spectroscopy of biological materials in the electron microscope. Nat. Commun. 7:10945 (2016)
