Multiple Hole Size


A single grid with a range of hole geometries is useful when carrying out cryo-electron tomography (cryo-ET) or where the sample size may vary unpredictably (for example liposomes).The predefined pattern of hole sizes and shapes in QUANTIFOIL® Multi A offers this possibility while still enabling automated data collection, in contrast to the random arrangements of traditional holey or lacey supports. 

Features include:

  • Oval holes which appear round in tomographic sections with high tilt angles (~70°).
  • Circular holes have diameters of 1 µm, 1.4 µm, and 2 µm.
  • Elliptical holes measure 1 µm x 4 µm and 2 µm x 8 µm.
Did you know?

We can offer Multi A on finder grids and alternative meshes to maximise their utility for tomography and integrated methods applications.


Uses of the Multi A format include:

  • Cryo-electron tomography, where the eliptical holes increase accessible tilt angle if aligned with the rotation axis. For example: Nedozralova et al. In situ cryo-electron tomography reveals local cellular machineries for axon branch development. J. Cell Biol. 221: e202106086 (2022). 
  • Larger particles and those of varying size. For example:

Koh et al. The structure of a 15-stranded actin-like filament from Clostridium botulinum. Nat. Commun. 10: 2856 (2019)

Rez et al. Damage-free vibrational spectroscopy of biological materials in the electron microscope. Nat. Commun. 7:10945 (2016)

Download list


Additional Ultrathin Carbon Layer

Improve particle dispersion and distribution across the entire grid by adding an ultrathin continuous carbon layer to most Quantifoil film supports.

Find out more