logo


QUANTIFOIL® with circular holes

QUANTIFOIL® with circular holes is mainly used in cryo-EM. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness.

The hole size that is chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. The latter makes assessment of the image quality easier, because the power spectrum of a foil is stronger than that of unsupported ice.

R 1/4
R 1/4
R 2/1
R 2/1
R 3.5/1
R 3.5/1

The QUANTIFOIL® types with small holes (e.g. 1.2 µm) were designed for magnifications around and higher 50.000x. At magnifications between 30.000x and 40.000x we recommend the QUANTIFOIL® types with 2 µm holes. Larger holes (e.g. 3.5 µm, 5 µm) are suitable at magnification around 20.000x.

The table below gives an overview of the available QUANTIFOIL® types with circular holes. Please be aware that the hole size and spacing might vary slightly from their nominal value, whereas the period is fix.

QUANTIFOIL® type

Hole size in µm

Spacing in µm

Period in µm

R 0.6/1

0.6

1.0

1.6

R 1/2

1.0

2.0

3.0

R 1/4

1.0

4.0

5.0

R 1.2/1.3

1.2

1.3

2.5

R 1.2/20

1.2

20.0

21.2

R 2/1

2.0

1.0

3.0

R 2/2

2.0

2.0

4.0

R 2/4

2.0

4.0

6.0

R 3/3

3.0

3.0

6.0

R 3/5

3.0

5.0

8.0

R 3.5/1

3.5

1.0

4.5

R 5/10

5.0

10.0

15.0

R 5/20

5.0

20.0

25.0

R 6/6.5

6.0

6.5

12.5

R 6/100

6.0

100.0

106.0

R 10/5

10.0

5.0

15.0

R 10/10

10.0

10.0

20.0

R 10/20

10.0

20.0

30.0

R 17/5

17.5

5.0

22.5